Be a Flow Cytometry Intracellular Staining Expert!
Staining for intracellular proteins, followed by flow cytometric analysis, provides useful information about a cell population that cannot be obtained by simple surface staining. With over a thousand researchers reaching out to us for assistance on intracellular staining each year, we want to help you become your lab's expert on staining intracellular antigens. Simply download the eBioscience Staining Intracellular Antigens for Flow Cytometry protocol today.
In this protocol, you will learn all about the following:
- Cytoplasmic intracellular proteins - a two-step staining protocol with permeabilization occurring after fixation. Ideal for detecting cytoplasmic proteins, cytokines and other secreted proteins.
- Nuclear intracellular proteins - a one-step staining protocol that combines fixation and permeabilization into a single step. Ideal for detecting transcription factors.
- Best practices on usage and handling of fluorochrome conjugated antibodies as well as links to additional flow cytometry resources on the web.
Download the protocol and be on your way to intracellular staining expertise!
